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Methylation is considered a key process in the synthesis of glutathione because part of the methylation cycle directly contributes to the production of cysteine, an essential amino acid needed to build glutathione molecules

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Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40