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The activation of these junctions may also induce long-term structural changes, such as the formation of tight associations with pericytes (which may encompass gene expression modulation), determining the stability and maturation of the vasculature [45], or short-lasting effects, like transient changes in endothelial permeability to solutes and circulating cells [46,47,48]

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3D), but also on more granular aspects of mouse behavior with each animal generating 2.5 million raw datapoints (30 FPS) over each 24-hour observation period (Fig