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Quantitation of bias Bias between cAMP, -arrestin-1, and -arrestin-2 responses was determined using a modified operational model of agonism
GSH-pull down assay of in vivo 35 S labeled viral proteins 250 l of in vivo 35 S labeled virus infected cell lysate (about one forth of total lysate harvested from 35 mm dish) was mixed with 100 l of GSH-sepharose 4B beads or Sepharose 4B Beads and subjected to a GSH-pull down assay
The number of treatment-emergent adverse events (TEAEs) was a secondary safety endpoint
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