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Group IV (Control-combination) was administered PBS for 3 days, then orally given a combination therapy of GABA (200 mg/kg/day for 21 days), probiotics (Fiber probiotic, Proflora, 0.2 g/kg/day for 21 days), vitamin D3 (SUN-D 1000, California Greens, 1,000 IU/kg/day for 14 days) and -lactam (Amoxicillin, HYMOX, 200 mg/kg/day for 7 days) as described in previous studies (Al-Suwailem et al., 2019)