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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

Modulation of GST P1-1 activity by polymerization during apoptosis

Slowly inject BAC water down the inside wall of the vial at an angle do not inject directly onto the peptide powder, as this can cause foaming and peptide degradation

Immunostaining revealed mixed cell populations with many undifferentiated hPSCs and mesoderm cells, and DE markers SOX17 and FOXA2 were expressed at lower levels compared to the Activin A-based control (CA)