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doi: 10.5114/wo.2013.33765 137 MatsuokaTYashiroM

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After cryosection (2030 m) and fixed for 10 min in 4% paraformaldehyde in PBS, sections were permeabilized for 10 min in 0.5% Triton (PBST) and blocked for 1 hr in a solution of 2% normal donkey serum, 1% BSA, and 0.3% Triton in PBS