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Methods Cell culture, expression constructs, and transfection MIN-6 (purchased from ATCC) cells and GLUTag cells (obtained from Takashi Tsuboi, the university of Tokyo, Japan) were cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum

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Potential interactions with insulin and other antidiabetic agents should be considered

Similarly, exenatide (another earlier GLP-1) showed no evidence for disease modification in a phase 3 Parkinsons disease trial