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RLS has been linked to genetic predispositions, iron deficiencies, and certain chronic conditions, such as diabetes or kidney disease

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Liquid vitamins must follow the 3-1-1 rule (3.4 oz or less per container)

Gut microbiota inoculum preparation from human feces for ex vivo study One gram of raw fecal sample was mixed with 10 mL of PBS supplement with 0.05% -cysteine in a tube containing glass beads, homogenized the mixture by vortexing for 1 min in an anaerobic chamber (Whitley DG250 Workstation, Don Whitley Scientific Limited, UK), filtered the homogenized fecal liquid through a Falcon 100 m cell strainer, and subsequently used the fecal filtrate for the study