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Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies were thoroughly eluted in stripping solution (Histova, Abcracker) for 60 min at 37 C
Am J Clin Nutr 69 : 467475
some studies determined dogs could not distinguish between shades of red, yellow, and green, while others have shown the opposite result
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