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3.3 Evaluation of cytokines and chemokines in tears during clinical study As detailed in the materials and methods section of the manuscript, the concentration of different cytokines and chemokines in tears was analyzed using flow cytometry at four study visits following randomization: V1, V3 (1 month after the initial control or the experimental formulation treatment), V4 (after the 1-week wash-out), and V6 (1 month after the crossover treatment)

however, some differences were noted

May reduce frequency of episodes over time

Transcript abundance was quantified with Kallisto, and differentially expressed genes (DEGs) were identified using DESeq2