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Mitochondrial biogenesis occurs continuously in healthy cells, as mitochondria undergo constant proliferation and fusion, but it can also be induced by oxidative stress, increased energy demands, physical activity, and certain diseases (45)

Macrophage production was initiated on day 10 by transferring the aggregated myeloid cell forming complexes onto an adherent 6-well tissue culture plate (TPP) and medium was changed to X-VIVO 15 (Lonza) containing 2 U/mL penicillinstreptomycin, 2 mM L-glutamine, 50 M -mercaptoethanol (all Gibco) supplemented with 25 ng/mL hIL-3 and 50 ng/mL human macrophage colony stimulating factor (hM-CSF, PeproTech)

1 Metabolic diseases like obesity and overweight have traditionally been treated with combinations of behavioral therapy and pharmacotherapy, the latter of which was often associated with poor efficacy and weight regain after medication cessation

As a powerful coenzyme found in every cell, NAD+ plays a key role in energy production, DNA repair, and slowing the effects of aging