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The chromosomes were then differentially stained without fluorochromes, following the method of Antoshchina and Poryadkova, allowing for clear differentiation of sister chromatids and accurate counting of exchange events [2]
All experiments were performed in triplicate
Injectable peptides require more care and knowledge of proper administration
Actually, many researchers agree that leptins main role is to signal starvation (via falling leptin levels) and consequently produce appropriate behavioral and metabolic responses that increase the chances of survival 27, 28
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