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Cells were fixed with 4% paraformaldehyde for 20 min at room temperature, and then permeabilized with 0.3% Triton X-100 for 10 min at room temperature

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Preliminary evidence suggests that a 12-week treatment with tirzepatide plus low-energy ketogenic therapy is more effective than its combination with a low-calorie diet in preserving fat-free mass, muscle strength, and resting metabolic rate in patients with obesity

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