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Some causes of B12 deficiency are: Pernicious Anemia Autoimmune Diseases Leaky Gut Syndrome Ulcerative Colitis, Crohns and Celiac Disease Small Intestinal Bacterial Overgrowth (SIBO) H Pylori Infection Prolonged Usage of Gastric Reflux meds Vegan and Vegetarian diets MTHFR gene mutations Bariatric Surgeries Excessive Alcohol Consumption B12 and the Methylation Cycle: Methylation is a critical process that happens trillions of times in every cell each minute

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For the endosomal recycling assay, dBCi cell monolayers were first biotinylated and then incubated at 37 C for 4 h with spike protein apically, or additional 20 h without Spike protein under ALI conditions

A., Kirch, J