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This leads to measurable increases in gonadotropins, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as gonadal steroids like testosterone (T) in male infants and estradiol (E2) in female infants [17,18,19]

Western Blot After the drug treatment, the proteins from the cultured SGNs were extracted with radioimmunoprecipitation assay buffer (RIPA) buffer (Protein Biotechnology, China)