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Whole blood samples were collected from the tail vein in 20-l heparinized capillary tubes (Fisher Scientific company LLC #22-362566), anesthesia was induced using isoflurane, the blood samples were transferred quickly (10 s) to 2-ml gas chromatographic septum sealed vials (Sigma #29381-U) containing 200 l of an internal standard solution consisting of 0.6 N perchloric acid and 4 mM n-propanol in double distilled water on ice