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Recombinant -synuclein and A1-40 activated the MAPkinase pathway in cells of hCMEC/D3 monolayers, so reducing expression of TJ proteins (Tai et al., 2010

SCFAs mainly include acetate, propionate, and butyrate, which are produced by the human gut microbiota through the fermentation of dietary fiber and amino acids obtained via hydrolysis using various pathways (18)

Temperature proves most critical - every 10C increase doubles degradation rate, meaning room temperature storage (25C) degrades peptides 4-8x faster than refrigeration (2-8C) and dramatically faster than freezing (-20C or below)