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To examine the mechanism of LaccID labeling, we generated a mutant in which the HCH motif, essential for electron transport 30 , was changed to three alanines

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I think we wanna understand a lot more about how these agents work, so I think thats why studies like this are important, but the ability to take a deeper dive beyond that will be critical

Finally, a corresponding horseradish peroxidase-conjugated secondary antibody was utilized to incubate the membrane for 2 h at room temperature