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They also introduced mutations that optimized the enzymes overall kinetics with the goal of driving the gluten peptide levels below the threshold for toxicity, which is estimated at 50 milligrams per day, and doing so within a physiologically relevant timeframe (7)

doi: 10.1016/j.lfs.2020.118986 83 LiangWZhouKJianPChangZZhangQLiuYet al

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Dried discs were transferred to 96 deep-well blocks and treated, while shaking, with side-chain deprotection solution, consisting of 90% trifluoracetic acid (TFA), 2% dichloromethane (DCM), 5% H 2 O and 3% triisopropylsilane (TIPS) (150 L/well) for 1.5 h at room temperature (rt)