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the increase in HO-1 expression induced by berberine is activated by Nrf2 activation through PI3K/AKT signaling pathway ( Studies have found that the combined treatment of DOX and berberine can significantly counteract the increase in acetylcholinesterase activity, oxidative stress, the decrease in glutathione content and CAT activity, and the increase in GFAP, NF-B and caspase-3 induced by DOX (Zhang et al., 2012)

Glutathione injections can be delivered into muscle (intramuscular) or fatty tissue under the skin (subcutaneous)

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