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An initial 4-month dose escalation period evaluated different doses (120, 240, and 360 g) and administration frequencies (two or three times daily) for pramlintide and was followed by an 8-month extension of the pre-assigned pramlintide treatment regime

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It should be noted that physiologic GLP-1R agonism in the periphery mediates food intake reduction via vagal afferent signaling as rats with a surgical subdiaphragmatic vagal deafferentation (SDA) exhibit a more mild food intake suppression in response to acute administration of GLP-1 analogues than sham operated animals (80, 81)

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