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A total of 17.6 l of the transfection mix was added to each well already containing 3.6 l siRNA (or 3.6 l water for mock controls), and the mixture was incubated at room temperature for 30 min

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The advantages include the absence of surgical intervention, low risk, and fewer complications, with most patients not experiencing recurrent leaks or fistulas after endoscopic treatment

PPP is regulated at the first enzyme, Glucose-6-Phosphate dehydrogenase (G6PD), activated when NADPH levels are low