The oral absorption of MTX is rapid but incomplete due to factors like receptor saturation, the inhibitory effect of food on its absorption, and rapid metabolization by gut flora (Attwa et al
To account for T 2 -weighting differences, PRESS water spectra were also collected using both echo times (echo time=122 or 68 ms, repetition time=2,000 ms, free induction decay points=2,048, spectral width=2,000 Hz, number of averages=8)
In the next day, 9 l of the drug dilutions (BSO, Auranofin, BSO+Auranofin) were added to the wells so that every drug/combination was tested at 5 (fixed concentration ratio, for combinations) concentrations (1:3 serial dilution) in 2 replicates
When to Reassess If injection-site reactions worsen instead of improving over the first week, if fatigue persists past the loading phase, or if any unusual symptom appears, pause the protocol and consult a qualified clinician