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Proteins were precipitated by Wessel/Flgge extraction 37 and pellets were dissolved in 100mM TEAB with endopeptidase LysC (2% w/w, enzyme/substrate) and incubated at 37C for 2-3 hours

Contraindications Do Not Use If You Have a known hypersensitivity to NAD+ or any component of the formulation Are pregnant or breastfeeding (safety has not been established) Have active cancer (theoretical concerns about supporting rapidly dividing cells) Warnings and Precautions Rate of administration: Rapid administration is associated with more intense side effects including chest tightness, shortness of breath, flushing, and nausea

The 25% Boost A major study found that NAD+ supplementation increased insulin sensitivity in skeletal muscle by 25% in prediabetic women