Slides containing retinal sections were deparaffinized by immersing the slides in various subsequent ethanol dilutions(100%, 95%,70% and 50%), then rinsed with phosphate-buffered saline, pH 7.4 (PBS, Quality Biological, 119-069-101), followed by incubation in blocking buffer containing 0.5% normal donkey serum (Abcam, ab7475), 0.5% bovine serum albumin (BSA, GoldBio, A-420-100), 0.1% Triton X-100 (Sigma, T8787-100) in PBS pH 7.4 for 2 h at room temperature 28
Proc Natl Acad Sci U S A 81:935939 Roberts CT Jr, Lasky SR, Lowe WL Jr, Seaman WT, LeRoith D (1987) Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues
Consider bland foods (e.g., crackers, toast, rice) if nausea occurs
1 Irrespective of the specific urate-lowering therapy (ULT) selected, fundamental principles entail commencing treatment concurrently with prophylaxis and initiating with a conservative dosage, followed by systematic monitoring of serum uric acid levels and subsequent dose adjustment until the therapeutic target is attained
