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EPR spectroscopy Continuous wave EPR spectroscopy was performed at 20 C with a X-band (9.6346 GHz) spectrometer (EMX-Nano, Bruker Biospin, with a cylindric cavity mode TM1110)

3.3 Environmental factors While diet and dietary additives such as pro-, pre and antibiotics play major roles in the formation of the gut microbiota, the water environment provides a complex and diverse living environment for aquatic animals with many important factors affecting the GM (Spanggaard et al., 2000)

This internal defense mechanism operates differently from hydrogen waters rapid, systemic free radical neutralization, offering a complementary approach to maintaining cellular health without one being inherently better than the other