But the technique still matters: the needle has to reach muscle, the site has to be clean, and the dose has to be right
Studies into the molecular mechanisms underlying the time of day differences post ischemia revealed that ischemic/reperfusion injury at ZT 18 had reduced infarct volume, edema, neurological deficits, apoptotic death, and improved neuronal survival compared to other time points (ZT0, ZT6, ZT12, and ZT18), coinciding with a decrease in PRAS40 and increased expression of Bmal1, Per1, Clock, AKT, Erk-1/2, mTOR, S6 , and BAD ( Disruptions to Circadian Rhythms Influence on Ischemic Stroke (Basic Science) Disruptions to circadian rhythms prior to ischemic events leads to a prothrombotic state resulting in a heightened predisposition for enhanced stroke damage and poor outcome
doi: 10.1038/srep46208
L.YamadaS.KagawaN.et al (2008)