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In addition, activation of Smad2/3 by NLRP3 phosphorylates vascular endothelial cells and transforms them into myofibroblasts, thereby increasing the production of -SMA and MMP9 [199]

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The brain of each mouse was then harvested, and 20 mg of tumor tissue was dissected for homogenization in 0.2 ml of lysis buffer, which is prepared by mixing two volumes of PBS with one volume of Stop buffer provided by the glucose uptake assay kit (Promega #J1342)