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The isolated cumulus cells were then resuspended in Histopaque 1077 (Sigma-Aldrich, Waltham, MA, USA) and enriched with a culture medium containing 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 5 mg/L insulin, 5 mg/L transferrin, 5 g/L sodium selenite (ITS, Sigma), and 1.25 M androstenedione (4-androstene-3, 17-dione, Sigma)

5 Conclusion This systematic review and meta-analysis indicate that sublingual and oral vitamin B12 administration provide efficacy comparable to intramuscular injection in improving serum cobalamin levels and reducing hyperhomocysteinaemia

Discussion In the present study, we used the Cre-loxP technique to remove IR from chondrocytes, which has enabled us to determine the contribution of insulin signaling through its cognate receptor