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2.18 Multiplex enzyme-linked immunosorbent assay profiling of TCM and NCM To assess angiogenic, vascular injury, pro-inflammatory, cytokine and chemokine secretions a 54-plex ELISA kit separated across 7 plates was used (Meso Scale Discovery, Rockville, MD, USA)

Crucially, the trial unmasked that this risk is not driven by weight loss alone

The SEMALEAN study (2025): "Semaglutide treatment resulted in a 15% reduction in visceral fat," reports a meaningful reduction in visceral fat during therapy, which is important for reducing metabolic risk