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[36] Systemic fungal infections represent another absolute contraindication, as corticosteroids may suppress immune function and could potentially worsen fungal infections

Subsequent small studies have produced inconsistent findings

Ethanol was removed, and the mixture was replaced with a buffer containing PBS (pH 7.5) containing 155 mM NaCl (S433750-100g, Aladdin, Shanghai, China), 3 mM Na2HPO4 (S118442-500g, Aladdin, Shanghai, China), and 1 mM KH2PO4 (P113042-500g, Aladdin, Shanghai, China) through dialysis

This change in food intake is largely mediated by estradiol, which acts directly upon anorexigenic and orexigenic neurons, largely in the hypothalamus