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The cells were subsequently received different treatments for 24 h and were cultured in a serum-free medium containing a labeling media, which consisted of BODIPY cholesterol (0.025 mM) (BODIPY-Cholesterol, Avanti Polar Lipids, Alabaster, AL, United States), MCD (10 mM, sigma), HEPES (Sigma), and egg phosphatidylcholine (0.1 mM) (Avanti Polar Lipids) ( g for 5 min the lysate supernatant was collected or fluorescence intensity determination

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Both T2DM and obesity are associated with a higher risk of cancer, which can be reduced by intentional weight loss, whereas effects of a reduction in hyperglycaemia are uncertain

S4DF) and F1 adult fish (refer to Fig