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Moreover, ferritinophagy is mediated by nuclear receptor coactivator 4 (NCOA4), which can be inhibited by HIF-1a and endothelial cell (EC)-derived extracellular vesicles (Exos) (36)

Gholizadeh-Ghaleh Aziz S, Fardyazar Z

After cryoprotection in 10% sucrose with 0.1 M phosphate buffer containing 0.01% sodium azide (VWR) and 0.02% bacitracin (Sigma) for 48 h, the tissue was embedded with OCT (HistoLab), frozen with liquid carbon dioxide and sectioned on a CryoStar NX70 cryostat (Thermo Scientific) at 12-m thickness