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Intracellular ROS scavenging and oxygen production DAF-FM DA probe for NO Raw 264.7 cells were cultured at a density of 7 10 4 cells per well in a 12-well plate for 24 h, and then incubated with PBS, PBS + Laser, 28.8 g/mL PDA, 40 g/mL PDA@Pt, and 40 g/mL PDA@Pt + Laser (1 W/cm 2 , 5 min) for another 24 h

Study population and eligibility criteria We reasoned to choose as patients cohort a group of healthy obese women with polycystic ovary syndrome (PCOS) that have increased metabolic risk, in order to avoid any other concomitant clinical feature as a confounding factor, and potentially affecting the results of our study