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For IGF-1R/InsR HybridR activation, IGF-1R immunoprecipitates were probed with an antibody against the InsR specific pTyr 1334 residue (#44809G, Invitrogen/ThermoFisher) 52 , and total HybridR determined by immunoblotting with an Anti-InsR antibody (#3025)

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Although there seemed to be more benefits, a large calorie restriction without increased physical activity was not an optimal strategy for physical fitness

Plata, A