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Harvested cells were resuspended in 40 ml lysis buffer (50 mM TrisHCl (pH 8.0), 10 mM MgCl 2 , 10% glycerol, 300 mM NaCl, 0.2 U ml 1 DNase I, 1 mM phenylmethylsulfonyl fluoride and complete proteinase inhibitor mixture (Roche)) and homogenized with an EmulsiFlex instrument (Avestin) for 20 min

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