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Bacterial cells were collected by centrifugation at 4,000 g for 20 min at 4 C and the cell pellet was resuspended in buffer (50 mM NaH 2 PO 4 and 300 mM NaCl, pH 6.0)

This activation triggers downstream signaling pathways, leading to the phosphorylation of STAT proteins, thereby regulating the expression of a series of genes and affecting cell function and physiological activity