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Semaglutide, tirzepatide, and retatrutide (Biosynth, Compton, UK, lot #s 0000042482, 0000054911, and 0000205204 respectively) were dissolved in 0.3% phosphate-buffered saline (PBS), which was adjusted to a pH of 8.0 using NaOH and injected subcutaneously (s.c.) at 1 ml/kg

cagrilintide + tirzepatide The cagrilintide dosage with tirzepatide combination targets three pathways (GLP-1, GIP, and amylin) versus four with the retatrutide combination (GLP-1, GIP, glucagon, and amylin)

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