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Immunoaffinity purification of acetylated peptides IMAC flowthrough fractions were concatenated into four fractions, reconstituted in immunoaffinity purification (IAP) buffer and incubated with anti-acetyllysine antibody beads (Cell Signaling PTMScan)

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PAHSA have been shown to improve glucose homeostasis in insulin-resistant obese mice as a result of enhanced insulin function in the liver leading to decreased hepatic glucose synthesis

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