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Accordingly, other routes such as sc or oral administration are recommended over ip injection.[4] IP injections should not be conducted in the same animal more than once daily as cumulative substance absorption following repeated dosing may prove fatal.[1, 4] Injected substances should be sterile, isotonic, and non-irritating.[7] Intraperitoneal Injection Technique in mice The animal should be restrained with its head and body tilted downwards to ensure the organs are out of the way when performing the injection.[1, 7, 8] An appropriately sized needle (Table 1) should be injected from the caudal direction, at a slight angle to the abdominal wall, with a quick and firm movement, but only so far that the tip barely pierces the abdominal wall

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