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Assessment of -amyloid protein, neurotropic markers, purinergic and cholinergic activities in hippocampus tissues Enzyme-linked immunosorbent assays (ELISA) were used to quantify several neural biomarkers in hippocampal tissue homogenates, including brain-derived neurotrophic factor (BDNF), insulin-like growth factor-1 (IGF-1), glycogen synthase kinase-3 beta (GSK3), beta-site amyloid precursor protein cleaving enzyme 1 (Bace1), adenosine triphosphatase (ATPase), adenosine deaminase (ADA), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE)

Reactive oxygen species: metabolism, oxidative stress, and signal transduction

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