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The mice were observed for clinical signs and mortality for 14 days at 0, 1, 2, 3 and 4 hours after dosing

The molecule was first isolated by Pickart in 1973 as the active component of human albumin fraction responsible for inducing older liver tissue to synthesize proteins characteristic of younger tissue an observation that led to subsequent decades of research into GHK-Cus effects on wound healing, skin regeneration, gene expression, and cellular senescence

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The burden is significantly higher in women than in men, with marked regional disparities (Huang et al., 2025