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Theriogenology 105, 150157
LY294002 and SP600125 were from EMD (Gibbstown, NJ, USA) Western blot analysis The antibodies used for western blotting included those against stress-activated protein kinase (SAPK)/JNK, phospho-SAPK/JNK (Thr183/Tyr185), caspase-7, caspase-9, phospho-Bcl-2 (Ser70), and poly(ADP-ribose) polymerase (PARP) (Cell Signaling Technology, Danvers, MA, USA), cytochrome c and -actin (Sigma, St Louis, MO, USA), cytochrome oxidase subunit IV (Cox IV
Storage and Handling Requirements To safeguard the complex structural integrity and prevent premature thermal or mechanical breakdown of the 20mg lyophilized cake, all vials must be stored long-term in a freezer environment at -20C, completely shielded from light and ambient moisture
For instance, oxidized AAT inhibits inflammation in response to cigarette smoke in vivo 155 and induces a broad anti-inflammatory profile in gene expression of primary human lung microvascular endothelial cells
