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The lyophilized TMT-labeled peptides were resuspended in TiO 2 loading buffer (80% acetonitrile (ACN), 5% trifluoracetic acid (TFA), 1 M glycolic acid) and incubated with 0.6 mg TiO 2 beads (GL Science, Japan) per 100 g peptide on a shaker for 10 min

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