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Quantitative real-time PCR (qRT-PCR) of mRNA and microbial DNA Total RNA from tissues was extracted using TRIzol reagent (Yamei, Shanghai, China) and reverse-transcribed into complementary DNA (cDNA) with a reverse transcription kit (Vazyme, Nanjing, China)

This includes the nucleus accumbens, ventral tegmental area, and prefrontal cortex areas implicated in substance use disorders

Frequently asked questions Can peptides replace antihistamines for allergies

In December 2024, this connection was officially recognized when tirzepatide (Zepbound) became the first FDA-approved medication for moderate to severe sleep apnea in adults with obesity