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Several small clinical trials and mechanistic studies suggest berberine may improve insulin sensitivity, reduce fasting glucose, and modestly lower lipid levels, though effect sizes are typically smaller than those observed with prescription medications

Grade Study Research Group, Nathan DM, Lachin JM, Balasubramanyam A, Burch HB, Buse JB, Butera NM, Cohen RM, Crandall JP, Kahn SE, Krause-Steinrauf H, Larkin ME, Rasouli N, Tiktin M, Wexler DJ, Younes N, The GSRG

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While the medication works continuously in your system regardless of when you inject, circadian rhythms and daily eating patterns mean that morning versus evening administration may produce different results for different people