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HbA 1c levels were assumed to increase at an annual rate of 0.14%, based on the drift seen in the ADOPT study [37]

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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)