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The supernatant was then analyzed by reverse-phase HPLC (Agilent 1260 Infinity LC, USA) on a Welch XB-C18 column (5 m, 250 mm 4.6 mm) equipped with a fluorescence detector at the excitation and emission wavelengths of 365 and 440 nm, respectively [9]

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This approach is an evolution of an older technique called dry needling

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