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Equine sperm Stuhtmann et al [9] and Heutelbeck et al [10,11] used similar two-layer gradients for stallion sperm of 1.090 and 1.165 g/ml or 1.090 and 1.170 g/ml and commented that these gradients promoted better retention of morphology and progressive motility and that the gradient purification was more effective if carried out just after collection rather then immediately before cryopreservation

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