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OPA-based assays are more sensitive but unstable, which affects accuracy and precision [86], whereas DTNB-based assays allow for determination of biothiols in the presence of other amino acids and polyphenolic antioxidants but are less sensitive [82]

Under these circumstances, BAP31 is cleaved with consequent formation of the p20 fragment, which activates the mitochondrial apoptotic events such as cytochrome c release, resulting in activation of the intrinsic apoptotic pathway [123, 125]